ABSTRACT Sanchez, Aerom Marz M. February 2023. SURVEILLANCE OF ANTIBIOTIC RESISTANCE IN ESCHERICHIA COLI THROUGH HOSPITAL SEWAGE WATER ANALYSIS, BACHELOR OF SCIENCE IN BIOLOGY MAJOR IN MICROBIOLOGY. Pam panga State Agriculturat University Adviser: Michael Angelo C. Nicdao, Ph.D. Escherichia coli (E col) is a Gram-negative bacterium that can be mostly found in water and in humnan microbiota and sometimes, on various food products. It is commensal by nature in the microbiota but becomes pathogenic when translocated to the environment. Therefore, E. coli is an indicator species for fecal contamination in water and food analyses. Infection from pathogenic E. coli usually comes from ingestion of contaminated water or food which causes a wide array of illnesses ranging from mild stomach pains to severe bloody diarrhea. The reproduction rate of E. coli is faster among other bacteria making them a reservoir for resistance genes within the environment. This makes them suitable candidates for surveying antibiotic resistance in sewage water. The study sought to create a preliminary antibiogram profile of E. coli isolated from the sewage water of Dr. Andres J. Luciano District Hospital in Magalang, Pampanga. The samples underwent a series of dilutions, incubation, and inoculation to various media to get the 42 E. coli isolates. The isolates were then subjected to minimum inhibitory concentration testing for antibiotic susceptibility test. The study found 45% resistance prevalence to amoxicillin with no susceptible isolates, 19% resistance prevalence in cefixime, and no resistance prevalence was observed in ciprofloxacin. Thirty-seven isolates were recalcitrant to at least two tested antibiotics and majority were recalcitrant to all three tested antibiotics (62%). The results confirm the presence of antibiotic resistance and possible extended-spectrum lactamases genes in E. coli within the clinical setting of Dr. Andres J. Luciano District Hospital. Key Words: Antibiotic Resistance, Antibiotic Suseptibility Testing, Escherichia coli, Recalcitrance, Sewage water.