Gayola, Angelica P. July 2022. PHYLOGENY OF 16S RIBOSOMAL RNA AND VIRULENCE GENES OF "ESKAPE" PATHOGENS: AN JIN-SILICO STUDY. BACHELOR OF SCIENCE IN BIOLOGY. Pampanga State Agricultural University. Adviser: Michael Angelo C. Nicdao, Ph.D. The "ESKAPE" pathogens are responsible for the majority of antibiotic resistance problems and hospitalizations worldwide. Phylogenetic analysis, a tool for determining the origins and early dissemination of a disease, has the potential to transform the battle against infectious disease outbreaks. Hence, the aim of this In-silico study was to examine the evolutionary relationship between /6S rRNA and the virulence genes of ""ESKAPE" pathogens acquired from NCBI GenBank. One hundred seventy eight sequences were obtained from nucleotide database. The sequences contain both the /6S rRNA and the specific virulence genes for each bacterium. They were aligned using BioEdit software and trees were constructed using MEGA 11. Phylogenetic trees revealed that the continent, country, human host disease, and year of bacterial isolation have no effect on DNA sequences of "ESKAPE" pathogens. 16S rRNA gene sequences did not cluster into groups similar to virulence gene sequences, regardless if both genes are from the same strain. Keywords: 16S rRNA gene, antimicrobial resistance (AMR), community-acquired infection (CAD), database, "ESKAPE" pathogens, healthcare-associated infection (HAD), NCBI GenBank, virulence genes, In-silico, phylogenetic relationship, sequence types (STs).