The study was conducted at Barangay San Agustin, Santa Rita, Pampanga, to determine the effect of mycorrhiza on hot pepper under different watering intervals. The study used Randomized Complete Block Design (RCBD) with three replications. The pot experiment comprised of main treatments, namely: MI (with mycorrhiza) and M2 (without mycorthiza); and days of watering intervals as sub-treatments: WI (three days watering interval), W2 (five days watering interval), and W3 (seven days watenng interval). Results of the study show that increasing watering interval negatively affects the root and vegetative growth of hot pepper. The days of watering intervals significantly influenced the plant height, length and diameter of roots, root to shoot ratio, and the number of secondary roots of the hot pepper. Treatments with three days watering interval were found to produce the tallest plants, longest and thickest primary roots, and the most number of secondary roots. However, Jowest root to shoot ratio was found at three days watering interval. On the other hand, mycorrhiza application has a positive influence on the root growth of hot pepper wherein treatments applied with mycorrhiza was observed to increase the number of secondary roots. It is recommended that farmers can apply mycorrhiza to enrich the number of secondary roots. Since days of watenng interval and mycorrhiza showed a significant effect on hot pepper, it is suggested that a study using similar treatments be conducted until the yield performance in order to provide more conclusive results.