Pampanga State Agricultural University

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The Morphological, Molecular Identifi-~ Cation And The Phylogeny Of Selected Varieties Of Sweet Potato In Department Of Agriculture Cliarc Tarlac (Record no. 15592)

MARC details
000 -LEADER
fixed length control field 02296ntm a2200169 a 4500
001 - CONTROL NUMBER
control field 134479
003 - CONTROL NUMBER IDENTIFIER
control field 0000000000
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250408094535.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 230201n 000 0 eng d
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Quiambao, Eunice C.
245 00 - TITLE STATEMENT
Title The Morphological, Molecular Identifi-~ Cation And The Phylogeny Of Selected Varieties Of Sweet Potato In Department Of Agriculture Cliarc Tarlac
Medium [manuscript] /
Statement of responsibility, etc. Eunice C. Quiambao.
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Magalang, Pampanga :
Name of publisher, distributor, etc. Pampanga State Agricultural University,
Date of publication, distribution, etc. April 2019.
300 ## - PHYSICAL DESCRIPTION
Extent viii, 107 leaves ;
Dimensions 28 cm. + 1 computer disc (4 3/4 in.)
505 0# - FORMATTED CONTENTS NOTE
Formatted contents note This study entitled "The Morphological, Molecular Identification and the Phylogeny of the Selected Varieties of Sweet Potato in Department of Agriculture CLIARC Tarlac" aimed to obtain the morphological characteristics of the species and the molecular identification of all the species (A-Fulfa Tarlac White (FTW), B-Orange Fleshed Sweet Potato (OFSP), C-Japonita, D-NSIC SP-32, E-Japanese Sweet Potato (SPJ), F-Taiwan Orange) using matK DNA markers; the relationship of the samples using Maximum Likelihood, Maximum parsimony and Neighbor Joining Tree. DNA Amplification was performed following the protocol of the angiosperms. The chloroplast maturase K gene (matK) is one of the most variable coding genes for angiosperm and has been suggested to be a "barcode" for land plants. Results show that the coding gene was amplified using matK DNA primer at 1570 bp. The species identity was recognized by their morphological and molecular characteristics. The purified sample were then sent to Macrogen, South Korea for DNA Sequencing. The raw DNA sequences were edited and assembled using Codon Code Aligner based on the peaks of the Consensus Sequences Chromatogram. The molecular identities of the samples were obtained based on BLAST Nucleotide Search results. They were identified as Ipomoea batata (AFulfa Tarlac White (FTW)99.43 % and 99.77 %, B-Orange Fleshed Sweet Potato (OFSP)99.54 %, C-Japonita 99.77%, D-=NSIC SP=-32 99.65%, E-Japanese Sweet Potato (SPJ)99.32 %, Fe Taiwan Orange99.43 %).
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Sanchez,Jacob Anderson C.,
Relator term Adviser.
Holdings
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      Not for loan BS Biology PSAU OLM PSAU OLM Dissertation, Theses 02/01/2023   UT Q6 2019 UT12401 04/08/2025 04/08/2025 Theses