Pampanga State Agricultural University

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Isolation of IgY From Yolks of Immunized Hens For the Prevention and Treatment of Canine Parvoviral Enteritis (Record no. 14515)

MARC details
000 -LEADER
fixed length control field 01723ntm a2200157 a 4500
001 - CONTROL NUMBER
control field 129824
003 - CONTROL NUMBER IDENTIFIER
control field 0000000000
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250408094147.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 210204n 000 0 eng d
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Tayag, Kier D.
245 00 - TITLE STATEMENT
Title Isolation of IgY From Yolks of Immunized Hens For the Prevention and Treatment of Canine Parvoviral Enteritis
Medium [manuscript] /
Statement of responsibility, etc. Kier D. Tayag.
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Magalang, Pampanga :
Name of publisher, distributor, etc. Pampanga State Agricultural University,
Date of publication, distribution, etc. 2020.
300 ## - PHYSICAL DESCRIPTION
Extent ix; 51 leaves + CD ;
Dimensions 28 cm.
500 ## - GENERAL NOTE
General note This study aimed to isolate canine parvovirus specific IgY from immunized white leghorn chicken eggs. Two chickens were immunized with a commercial canine parvovirus vaccine for five weeks at weekly interval. Sera from immunized chickens were collected to confirm the immune response to the vaccine. Presence of antibody against canine parvovirus was checked using indirect adsorption Enzyme-Linked Immunosorbent Assay (ELISA). The tires of sera collected from the two hens five weeks after immunization were found to have 3.45 and 3.33 absorbance units (Au), respectively. Eggs from immunized chickens were also collected after the immunization period. Egg yolks were separated and processed for IgY isolation using the optimized protocol (sodium chloride method). A total of 450 mg of purified IgY was isolated from four immunized eggs. Immunoreactivity of the isolated IgY was tested using indirect adsorption ELISA. The ELISA titer of the isolated canineparvovirus-specific IgY was found to have 2.91 Au, suggesting that the isolated IgY were specific for canine parvovirus.
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      Not for loan PSAU OLM PSAU OLM Dissertation, Theses 02/04/2021   UT T23 2020 UT12025 04/08/2025 04/08/2025 Theses