DNA-Based Assay of Pathogenic Vibrio parahaemolyticus From Cooked Crabs in Mabalacat City (Record no. 14439)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02004ntm a2200157 a 4500 |
| 001 - CONTROL NUMBER | |
| control field | 129744 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | 0000000000 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250408094144.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 210121n 000 0 eng d |
| 100 0# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Cabuguason, Micah O. |
| 245 00 - TITLE STATEMENT | |
| Title | DNA-Based Assay of Pathogenic Vibrio parahaemolyticus From Cooked Crabs in Mabalacat City |
| Medium | [manuscript] / |
| Statement of responsibility, etc. | Micah O. Cabuguason. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Magalang, Pampanga : |
| Name of publisher, distributor, etc. | Pampanga State Agricultural University, |
| Date of publication, distribution, etc. | 2020. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | ix; 51 leaves + CD ; |
| Dimensions | 28 cm. |
| 500 ## - GENERAL NOTE | |
| General note | Vibrio parahaemolyticus is one of the leading causes of seafood-borne gastroenteritis and now considered as an emerging food-borne illness worldwide, following consumption of raw, undercooked or mishandled marine products. Besides gastroenteritis, Vibrio parahaemolyticus also causes wound infection or septicemia in individuals with pre-existing medical conditions. The recent increased demand and consumption of marine products especially crabs and the proliferation of seafood restaurants around Pampanga, creates higher food safety risks from said pathogen. Therefore, this study aimed to determine the presence of pathogenic Vibrio parahaemolytic in crabs collected from randomly selected seafood restaurants in Mabalacat City, Pampanga. The DNA samples were extracted from the cooked tissue of five (5) individual crabs. The samples were subjected to nucleic acid purity quantification and polymerase chain reaction (PCR) assays. The PCR products were further analyzed and visualized through gel electrophoresis and UV transilluminator, respectively. Results from the spectrophotometer yielded an approximate analysis of l.122 A260/A280 nucleic acid purity ratio by a software and showed no genetic material of the Vibrio parahaemolyticus from the gel electrophoresis. Thus, this study showed that the purchased cooked crab dish were safe for human consumption. |
| Withdrawn status | Lost status | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Total checkouts | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Not for loan | PSAU OLM | PSAU OLM | Dissertation, Theses | 01/21/2021 | UT C11 2020 | UT11979 | 04/08/2025 | 04/08/2025 | Theses |