Pampanga State Agricultural University

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Utilization of Aqueous Plant Extracts As Stains For Gonad Examination of Nile Tilapia (Record no. 14428)

MARC details
000 -LEADER
fixed length control field 01910ntm a2200157 a 4500
001 - CONTROL NUMBER
control field 129733
003 - CONTROL NUMBER IDENTIFIER
control field 0000000000
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250408094144.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 210121n 000 0 eng d
100 0# - MAIN ENTRY--PERSONAL NAME
Personal name Baltazar, Kristine Joyce M.
245 00 - TITLE STATEMENT
Title Utilization of Aqueous Plant Extracts As Stains For Gonad Examination of Nile Tilapia
Medium [manuscript] /
Statement of responsibility, etc. Kristine Joyce M. Baltazar.
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Magalang, Pampanga :
Name of publisher, distributor, etc. Pampanga State Agricultural University,
Date of publication, distribution, etc. 2020.
300 ## - PHYSICAL DESCRIPTION
Extent xi; 58 leaves + CD ;
Dimensions 28 cm.
500 ## - GENERAL NOTE
General note The study was conducted to determine and compare the staining effect of C. longa, I. batatas and M. acuminata aqueous extracts for gonad examination of nile tilapia. Twelve nile tilapia fingerlings with approximately two and one-half to four-inch size were divided into four treatments with three replicates each. Treatment 1 was assigned as the positive control, which were stained with malachite green, while Treatments 2, 3 and 4 were stained with C. longa, I. batatas and M. acuminata extracts, respectively. The prepared slides of stained gonads were randomly distributed on the microscopes using completely randomized design (CRD)> Blind examination and evaluation by five faculty members was done on the stained unlabeled tissues under the microscopes. The tissues were evaluated according to five parameters: hue. saturation, contrast, clarity and overall appearance. A scoring scheme was followed, i.e. 1 to 2 - very poor; 3 to 4 - poor; 5 to 6 - average; 7 to 8 - good; and 9 to 10 - excellent. Data gathered were subjected to one way analysis of variance (ANOVA). The results suggested that C. longa, I. batatas and M. acuminata extracts are potential alternative stains for gonadal examinations of nile tilapia with M. acuminata as the most preferred.
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      Not for loan PSAU OLM PSAU OLM Dissertation, Theses 01/21/2021   UT B19 2020 UT11974 04/08/2025 04/08/2025 Theses