Cytotoxicity Screening And Anthelminthic Potential Of Glinus Oppositifolius Extract Against Rumen Flukes
Negre, Kristene Joy C.
Cytotoxicity Screening And Anthelminthic Potential Of Glinus Oppositifolius Extract Against Rumen Flukes [manuscript] / Negre, Kristene Joy C. - Magalang, Pampanga : Pampanga State Agricultural University, May 2023. - xvii, 82 leaves ; 28 cm + 1computer disc (4 3/4 inch)
ABSTRACT NEGRE, Kristene Joy C. May 2023. CYTOTOXICITY SCREENTNG AND ANTHELMINTHIC POTENTIAL OF Glinus oppositifolius EXTRACT AGAINST RUMEN FLUKES. BACHELOR OF SCIENCE IN BIOLOGY MAJOR IN MOLECULAR BIOLOGY AND BIOTECHNOLOGY. Pampanga State Agricultural University, Magalang, Pampanga. Adviser: Cristel Joy C. Mallari, M.Sc. Helminthiasis in livestock threatens its production and profitability. An increase in the development of resistance in the parasites is one of the most challenging concerns related to the treatment of the disease, necessitating therapeutic alternatives from plant-derived natural products. Hence, the study investigated the cytotoxicity and anthelmintic potential of Glimus oppositifolius ethanolic and aqueous extracts against rumen flukes through the brine shrimp lethality assay (BSLA) and adult motility assay (AMA). Four concentrations of G. oppositifolius ethanolic and aqueous extracts, 10,000 ppm, 1,000 ppm, 100 ppm, and 10 ppm were used in BSLA in comparison with the positive control (ethanol) and negative control (artificial seawater). As such, the AMA was assessed using three concentrations of each extract are as follows: 97.39 ppm, 194.78 ppm, and 292.17 ppm. Albendazole was used as a positive control, and nutrient broth was used as a negative control. Results showed that both extracts exhibited toxicity, with a median lethal concentration (LCs0) of 0.039 ppm (ethanolic) and 54.80 ppm (aqueous) after 24 hours. For AMA, the results revealed that the highest concentration of the ethanolic extract was comparable to the positive control, with 100% inhibition after 12 hours. Thus, both extracts showed significant differences (pK0.05) from the negative control. Additionally, the LCS0 for both extracts were 95.58 ppm (ethanolic) and 145.57 ppm (aqueous). In conclusion, all treatments exhibited significant cytotoxic and anthelminthic activity in a time- and dose-dependent manner. Findings also implied that ethanolic extract was more lethal than aqueous extract as determined in both assays (BLSA and AMA). Therefore, the use of Glinus oppositifolius extract as a plant-based anthelminthic agent is a viable option to combat rumen flukes. Keywords: Glinus oppositifolius, rumen flukes, cytotoxicity, anthelminthic.
Cytotoxicity Screening And Anthelminthic Potential Of Glinus Oppositifolius Extract Against Rumen Flukes [manuscript] / Negre, Kristene Joy C. - Magalang, Pampanga : Pampanga State Agricultural University, May 2023. - xvii, 82 leaves ; 28 cm + 1computer disc (4 3/4 inch)
ABSTRACT NEGRE, Kristene Joy C. May 2023. CYTOTOXICITY SCREENTNG AND ANTHELMINTHIC POTENTIAL OF Glinus oppositifolius EXTRACT AGAINST RUMEN FLUKES. BACHELOR OF SCIENCE IN BIOLOGY MAJOR IN MOLECULAR BIOLOGY AND BIOTECHNOLOGY. Pampanga State Agricultural University, Magalang, Pampanga. Adviser: Cristel Joy C. Mallari, M.Sc. Helminthiasis in livestock threatens its production and profitability. An increase in the development of resistance in the parasites is one of the most challenging concerns related to the treatment of the disease, necessitating therapeutic alternatives from plant-derived natural products. Hence, the study investigated the cytotoxicity and anthelmintic potential of Glimus oppositifolius ethanolic and aqueous extracts against rumen flukes through the brine shrimp lethality assay (BSLA) and adult motility assay (AMA). Four concentrations of G. oppositifolius ethanolic and aqueous extracts, 10,000 ppm, 1,000 ppm, 100 ppm, and 10 ppm were used in BSLA in comparison with the positive control (ethanol) and negative control (artificial seawater). As such, the AMA was assessed using three concentrations of each extract are as follows: 97.39 ppm, 194.78 ppm, and 292.17 ppm. Albendazole was used as a positive control, and nutrient broth was used as a negative control. Results showed that both extracts exhibited toxicity, with a median lethal concentration (LCs0) of 0.039 ppm (ethanolic) and 54.80 ppm (aqueous) after 24 hours. For AMA, the results revealed that the highest concentration of the ethanolic extract was comparable to the positive control, with 100% inhibition after 12 hours. Thus, both extracts showed significant differences (pK0.05) from the negative control. Additionally, the LCS0 for both extracts were 95.58 ppm (ethanolic) and 145.57 ppm (aqueous). In conclusion, all treatments exhibited significant cytotoxic and anthelminthic activity in a time- and dose-dependent manner. Findings also implied that ethanolic extract was more lethal than aqueous extract as determined in both assays (BLSA and AMA). Therefore, the use of Glinus oppositifolius extract as a plant-based anthelminthic agent is a viable option to combat rumen flukes. Keywords: Glinus oppositifolius, rumen flukes, cytotoxicity, anthelminthic.