Nile tilapia is one of the most important freshwater species due to its widely culture and fast growth performance in aquaculture. However, transportation of live fish such as fingerlings is a very critical process due to several factors. Thus, it can cause to reduce the level of water quality, which results in psychological stress and increase the mortality of fish stock. Chemical additives in water are widely use to minimize the stress response of fish and herbal extract are recently investigating as alternative than synthetic drugs. This study aims to determine the effect of tamarind leaf extract as water additive in transporting of tilapia fingerlings. Based on the result there is no bioactive compounds found on the leaves extract. The obtained result on the survival showed that T4 (Dried leaves with 3g) and T9 (Fresh leaves with 9g) and T21 (combination of Na Cl and Fresh leaves with 9g) treatments got the highest survival rate of 100°o significantly after the transport duration. Meanwhile the result obtained in temperature on dried leaves, were range from (27.8 to 28.7° C) while fresh leaves were range from (28.8 to 30.6 ° C) respectively. In addition, the combination groups were range from (25.5 to 29.7 °C) and the controls group were range from (25.3 to 26.9 ° C) respectively. Statistically did not show the significant difference among the treatments (p<0.05) in temperature. Moreover, the pH result on dried and fresh leaves were significant difference (p<0.05) with the range of (7.8 to 8.0) respectively. Dissolved Oxygen in dried leaves were range from (0.4 to 0.9 ppm) while the fresh leaves were range from (0.4 to 1.4 ppm) significantly lower compared to combination groups. Fecal discharge, statistically revealed significant difference (p<0.05) in each other treatments. The result observed that dried leaves treatments ranging from (0.36 to 0.94g) had some higher feces discharge than fresh leaves treatments ranging from (0.04 to 0.44) he present study indicates that the used of tamarind leaf extract has a potential as stres litigator for transporting tilapia fingerlings.